---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-07-12 03:32:17+00:00
layout: post
slug: pcr-colony-pcr-on-restreaked-pgs2-clones-from-20110707
title: PCR - Colony PCR on Restreaked PGS2 Clones from 20110707
categories:
  - 2011
  - Miscellaneous
tags:
  - colony screen
  - COX
  - COX2
  - cyclooxygenase
  - gel
  - PCR
  - PGS
  - PGS2
  - prostaglandin synthase
---

Ran a colony PCR at the same time that I inoculated liquid cultures, using M13 primers.

Cycling params:




    
  * 94C - 10m



40 cycles of:


    
  * 94C - 1m

    
  * 50C - 1m

    
  * 72C - 2m



Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20110712-01.jpg)

Lane 1: Hyperladder I

Lane 2: PGS Lo 1

Lane 3: PGS Hi 3

Lane 4: PGS Hi 4

Lane 5: Neg. Control

The only colony with an insert is PGS Hi 4. Will run a plasmid prep. However, this is the same sample that was sent for sequencing that produced nothing but vector sequence...