---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-05-11 20:26:41+00:00
layout: post
slug: reverse-transcription-hard-clam-gill-dnased-rna-from-20110509
title: Reverse Transcription - Hard Clam Gill DNased RNA (from 20110509)
categories:
  - 2011
  - Miscellaneous
tags:
  - cDNA
  - DNased RNA
  - Hard clam
  - M-MLV
  - Mercenaria mercenaria
  - reverse transcription
---

Performed reverse transcription on DNased RNA from the hard clam vibrio tubiashii challenge experiment (see Dave's Notebook 5/2/2011), following the Promega M-MLV RT protocol with ~1ug of DNAsed RNA. [Master mix calcs are here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20110511-01.jpg). Reactions were done in a plate. cDNA was diluted 1:4 with H2O.
