---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-05-09 20:35:14+00:00
layout: post
slug: dnase-hard-clam-gill-rna-from-earlier-today
title: DNase - Hard Clam Gill RNA (from earlier today)
categories:
  - 2011
  - Miscellaneous
tags:
  - DNase
  - DNased RNA
  - Hard clam
  - Mercenaria mercenaria
  - NanoDrop1000
  - RNA
  - RNA quantification
  - Turbo DNA-free
---

DNased 5ug of RNA from each sample with Ambion's Turbo DNA-free Kit, according to the manufacturer's rigorous protocol. Samples were spec'd and stored @ -80C in the "Hard Clam V.t. Experiment RNA" box.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20110511%20DNased%20Hard%20Clam%20Gill%20RNA%20ODs.jpg)

Due to lack of a positive control (and lack of primers known to amplify gDNA), these DNased RNA samples will NOT be checked to verify elimination of gDNA carryover at this time. Will proceed with making cDNA for qPCR anyway.