---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-04-25 20:57:56+00:00
layout: post
slug: colony-pcr-colonies-from-cox1-genomic-cloning-from-20110411
title: Colony PCR - Colonies from COX1 Genomic Cloning (from 20110411)
categories:
  - 2011
  - Miscellaneous
tags:
  - colony screen
  - COX
  - COX1
  - Crassostrea gigas
  - cyclooxygenase
  - gel
  - Pacific oyster
  - PCR
  - prostaglandin synthase
---

Ran colony PCR on various colonies produced from cloning on 20110411. All colonies were picked, re-streaked on Kan50 plate(s) and PCR'd. [Master mix calcs are here](https://eagle.fish.washington.edu/Arabidopsis/20110425-01.jpg). Cycling params:




    
  * 95C - 10mins



40cycles of:


    
  * 95C - 30s

    
  * 55C - 30s

    
  * 72C - 3mins

    
  * 72C - 10mins



Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20110426-01%20Gel.jpg)
