--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2011-03-21 22:06:39+00:00 layout: post slug: qpcr-c-gigas-bbdh-cdna-for-props-timp3bb-primers title: qPCR - C.gigas BB/DH cDNA for PROPS (TIMP3(BB) primers) categories: - 2011 - PROPS tags: - BB - cDNA - CFX96 - Crassostrea gigas - DH - Pacific oyster - qPCR - TIMP3 --- Performed qPCR using cDNA from [20110311](/Sam%27s+Working+Notebook+Jan+2011+-+March+2011#sjw20110311). This was performed for 2 reps with TIMP3(BB) (SR IDs: 1067 & 1106). [Master mix calcs are here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20110321-02.jpg). Plate layout, cycling params, etc. can be found in the qPCR Report (see Results). Results: [qPCR Report](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Roberts%20Lab_2011-03-21%2013-52-24_CC009827.pdf) (PDF) [qPCR Data File](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Roberts%20Lab_2011-03-21%2013-52-24_CC009827.pcrd) (CFX96)