---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-03-11 23:15:51+00:00
layout: post
slug: reverse-transcription-c-gigas-bbdh-dnased-rna-from-20090507-for-props
title: Reverse Transcription - C.gigas BB/DH DNased RNA (from 20090507) for PROPS
categories:
  - 2011
  - PROPS
tags:
  - BB
  - cDNA
  - Crassostrea gigas
  - DH
  - DNased RNA
  - M-MLV
  - Pacific oyster
  - reverse transcription
---

Performed RT on DNased RNA using Promega MMLV RT and Oligo dT according to manufacturer's protocol, using 1ug of DNased RNA, but in a 50uL reaction. Due to large number of samples, cDNA was made in PCR plate. [Plate layout and calcs are here](https://spreadsheets3.google.com/ccc?key=tnCFOYm03SNc053JcCp75EQ&authkey=CKbw8YYG#gid=0).
