---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-03-01 23:39:34+00:00
layout: post
slug: nanodrop1000-comparison-roberts-vs-young-lab
title: NanoDrop1000 Comparison - Roberts vs. Young Lab
categories:
  - 2011
  - Miscellaneous
tags:
  - NanoDrop1000
  - troubleshooting
---

A previous comparison was performed (see 20110209), but it was determined that the standard DNA being used to test the machines was old/degraded. Lisa ordered a new standard DNA dilutions series (Quant-iT dsDNA Kit; Invitrogen) and these DNAs were used. All DNAs were measured 5 times and were mixed by gently flicking between each measurement. A "blank" was measured between each different [DNA] and, if the reading was > + or - 1ng/uL, the machine was reblanked.

Results:

Quick assessment is that Graham's NanoDrop1000 is more accurate than ours.

[Here is a spreadsheet with averages, standard deviations and experimental error (%)(https://spreadsheets4.google.com/ccc?hl=en&key=tSqYT6UZDXFLvxIq0XmCzFw&authkey=CICJ1-wC&hl=en#gid=0). Below are the raw measurements from both machines.

Roberts Lab ND100:

![](https://eagle.fish.washington.edu/Arabidopsis/20110301%20Roberts%20Lab%20Quant-iT%20Standards.jpg)

Young Lab ND1000:

![](https://eagle.fish.washington.edu/Arabidopsis/20110301%20Young%20Lab%20Quant-iT%20Standards.JPG)