---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-02-25 23:48:14+00:00
layout: post
slug: ethanol-precpitation-dnased-rna-bb01-from-earlier-today
title: Ethanol Precpitation - DNased RNA BB01 (from earlier today)
categories:
  - 2011
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DNased RNA
  - EtOH precipitation
  - Pacific oyster
---

Due to residual gDNA contamination, will EtOH precipitate in order to treat with DNase again. Add 0.5 vols 3M NaAOc (pH=

5.2), 2.5 vols 100% EtOH, mixed and incubated @ -20C for 30mins. Pelleted RNA @ 16,000g, 4C 30mins. Washed RNA with 1mL 70% EtOH (2x due to fear of residual salts from DNase Buffer). Pelleted RNA @ 16,000g, 4C, 15mins. Resuspended RNA in 45uL nuclease-free H2O. Sample was stored @ -80C (in "Sam's RNA Box #1) until it could be DNased again.
