---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-02-25 23:44:41+00:00
layout: post
slug: dnase-c-gigas-bb01-from-20110216
title: DNase - C.gigas BB01 from 20110216
categories:
  - 2011
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DNase
  - DNased RNA
  - NanoDrop1000
  - Pacific oyster
  - RNA quantification
  - Turbo DNA-free
---

Used EtOH precipitated BB01 RNA from [20110216](/Sam%27s+Working+Notebook+Jan+2011+-+March+2011#sjw20110216) and followed Ambion's "rigorous" protocol, utilizing a total of 2uL of DNAse. Briefly, samples were incubated @ 37C for 30mins, an additional 1uL of DNase was added to each sample, mixed and incubated for an additional 30mins @ 37C. After finishing protocol, samples were spec'd.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20110225%20DNased%20RNA.JPG)

First reading had an air bubble and should be ignored. DNased RNA looks good, based on 260/280 ratios. As is usually the case for DNased RNA, the 260/230 ratios are on the low side. Will check DNased RNA for residual gDNA.