---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-02-09 04:33:50+00:00
layout: post
slug: qpcr-test-young-lab-qpcr-calibration-repeat
title: qPCR - Test Young Lab qPCR Calibration (Repeat)
categories:
  - 2011
  - Miscellaneous
tags:
  - ABI 7300
  - qPCR
  - troubleshooting
  - Young Lab
---

This is a repeat of a run from 20110204. Here're master mix calcs. This was being repeated to evaluate whether or not the relative differences in Ct values observed on 20110204 are consistent or not across the plate. Cycling params were as follows:




    
  * 95C - 10min



40 Cycles of:


    
  * 95C - 15s

    
  * 55C - 30s

    
  * 72C - 1m



Melt curve.

Results:

Absolutely no amplification of any kind! Bizarre. Will repeat.