---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-01-29 04:50:11+00:00
layout: post
slug: dnase-dnase-c-gigas-rna-from-20110120-20110121-and-20110124
title: DNase - DNase C.gigas RNA from 20110120, 20110121 and 20110124
categories:
  - 2011
  - Miscellaneous
tags:
  - Crassostrea gigas
  - digestive gland
  - DNase
  - DNased RNA
  - gill
  - gonad
  - mantle
  - NanoDrop1000
---

5ug of RNA was DNased using Ambion's Turbo DNA-free kit, following the rigorous protocol (0.5uL of DNase for 30 mins then additional 0.5uL of DNase for 30mins). [Calcs for DNase reactions are here](https://spreadsheets.google.com/ccc?key=0AmS_90rPaQMzdEYzZEFjSXlGRUgzVUZBekpzZTMybmc&hl=en&authkey=CJ6rp5cM). RNA was stored @ -80C in Shellfish RNA Boxes [4](https://spreadsheets.google.com/ccc?key=0AmS_90rPaQMzcHdyU1d0MDVMLWpaTWdadnJSd0M4UUE&hl=en#gid=3) and [5](https://spreadsheets.google.com/ccc?key=0AmS_90rPaQMzcHdyU1d0MDVMLWpaTWdadnJSd0M4UUE&hl=en#gid=4). Samples will be spec'd on Monday.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20110131%20DNased%20RNA%20ODs.JPG)

Overall, the RNA looks really good (based on OD 260/280 numbers). Not surprisingly, the OD 260/230 values for all samples dropped, likely due to the addition of the buffer (salts) used in the DNase reaction. Emma says she will check these samples for residual DNA.

--UPDATE (20110131)-- Emma checked all DNased RNA samples on 20110131 using C.gigas 18s primers (SR ID: ?). She has not listed the results of the whether or not all samples are clean or if some still have residual gDNA carryover.

--UDPATE (20110201-- Samples that appear to have residual gDNA carryover based on Emma's qPCR on 20110131: Muscle C6, Gill 1hr C2 & E2.