---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2011-01-25 04:53:38+00:00
layout: post
slug: rna-isolation-various-c-gigas-tissue-from-20110111
title: RNA Isolation - Various C.gigas Tissue from 20110111
categories:
  - 2011
  - Miscellaneous
tags:
  - Crassostrea gigas
  - digestive gland
  - gonad
  - mantle
  - NanoDrop1000
  - Pacific oyster
  - RNA isolation
  - RNA quantification
  - TriReagent
---

RNA was isolated in 1mL TriReagent, according to protocol. Samples were resuspended in 50uL 0.1% DEPC-H2O and spec'd. RNA was stored @ -80C in "[Shellfish RNA Box #4](https://spreadsheets0.google.com/ccc?hl=en&key=pwrSWt05L-jZMgZvrRwC8QA&hl=en#gid=3)

Results:



# ![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20110124%20RNA.JPG)



RNA looks OK. Not surprising, but mantle and Dg/Gonad tissues ended up with poor OD260/230 ratios. This has been observed in the past with these tissue types.