---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-11-23 04:36:08+00:00
layout: post
slug: restriction-digestions-hpaii-and-mspi-on-macs-c-gigas-gdna-samples-round-1
title: 'Restriction Digestions - HpaII and MspI on Mac''s C.gigas gDNA Samples: Round
  1'
categories:
  - 2010
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNA
  - gill
  - gonad
  - HpaII
  - MspI
  - Pacific oyster
  - restriction digestion
---

Set up restriction digests for subsequent analysis by methylation specific PCR (MSP). This will be the first of two rounds of digestion with the same enzyme on each sample. [Samples and master mixes are here](https://spreadsheets.google.com/ccc?key=to7CQ1otBUzv34826s53JiA&hl=en&authkey=CKqciPYM#gid=0). Samples were incubated 3hr. @ 37C. All samples were heat inactivated at 80C for 30mins and then stored @ -20C.