---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-09-09 04:10:51+00:00
layout: post
slug: reverse-transcription-dnased-hard-clam-rna-from-earlier-today
title: Reverse Transcription - DNased Hard Clam RNA from earlier today
categories:
  - 2010
  - Miscellaneous
tags:
  - CA
  - cDNA
  - DNased RNA
  - Hard clam
  - M-MLV
  - MA
  - MAX
  - Mercenaria mercenaria
  - oligo dT
  - reverse transcription
---

Prepared cDNA using 1ug of RNA from each of the 3 pools (CA, MA, MAX) and processed according to Promega's M-MLV protocol, using oligo dT primers. [Calcs and master mix set up are here](https://spreadsheets.google.com/ccc?key=0AmS_90rPaQMzdEVLYTlhWlA3M0V0Y0luNzh3bU85SVE&hl=en&authkey=CKvPlt8G). Briefly, RNA was combined with oligo dT primers, denatured @ 70C for 5mins, immediately placed on ice for 2mins, mixed with RT master mix, incubated 1hr @ 42C, 3mins @ 95C, and then stored @ -20C.