--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2010-09-08 04:14:03+00:00 layout: post slug: rna-isolation-hard-clam-tissues-recd-from-rutgers-on-20100820 title: RNA Isolation - Hard Clam Tissues Rec'd from Rutgers on 20100820 categories: - 2010 - Miscellaneous tags: - CA - Hard clam - MA - MAX - Mercenaria mercenaria - NanoDrop1000 - RNA isolation - RNA quantification - Rutgers - TriReagent --- RNA was isolated from the following samples using TriReagent, according to protocol: MAX 1, 2, & 3 CA 1, 2, & 3 MA 1, 2, & 3 Samples were resuspended in 50uL of 0.1% DEPC-H2O and spec'd: ![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20100907-01%20RNA.JPG) Results: 260/280 ratios are decent for most of the samples, with MAX1 and MAX 2 being the exception. Both of these samples also have very poor 260/230 ratios. Out of curiosity, I will EtOH precipitate all samples to see if I can improve both the 260/280 and 260/230 ratios.