---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-09-08 04:18:23+00:00
layout: post
slug: etoh-precipitation-hard-clam-rna-from-earlier-today
title: EtOH Precipitation - Hard Clam RNA from earlier today
categories:
  - 2010
  - Miscellaneous
tags:
  - CA
  - EtOH precipitation
  - Hard clam
  - MA
  - MAX
  - Mercenaria mercenaria
  - NanoDrop1000
  - RNA quantification
---

RNA was mixed with 0.1 vols of 3M NAOAc (pH = 5.2) and 2.5 vols of 100% EtOH, vortexed and incubated @ -20C for 30mins. RNA was pelleted @ 16,000g, 30mins, 4C. Supe was discarded and pellet was washed with 1mL 70% EtOH. RNA was pelleted @ 16,000g, 15mins, 4C. This was step was repeated a second time. Supe was discarded, the RNA was resuspended in 50uL of 0.1% DEPC-H2O, spec'd and stored @ -80C:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20100907-02%20RNA.JPG)

Results:

Interestingly, precipitating the samples vastly improved the 260/230 ratios. However, the 260/280 ratios DECREASED for all samples except one (CA 1). Not really thrilled about this fact, nor am I sure why this would happen.

RNA was stored @ -80C in "Sam's RNA Box #1."