---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-07-09 04:39:54+00:00
layout: post
slug: gdna-isolation-various-gigas-samples
title: gDNA Isolation - Various gigas samples
categories:
  - 2010
  - Miscellaneous
tags:
  - 5-azacitidine
  - Crassostrea gigas
  - DNA Isolation
  - DNazol
  - gDNA
  - gill
  - gonad
  - mantle
  - Pacific oyster
  - Vibrio exposure
  - Vibrio tubiashii
---

Placed ~20mg fragments of tissue in 250uL DNAzol. Added 1.35uL of Proteinase K (Fermentas; 18.5mg/mL) to reach a final concentration of 100ug/mL. Incubated RT, O/N, end-over-end rotation. Will complete DNA isolation tomorrow.

Sample List:

Vt Gigas Live #3 Gill 24E (from [20080828; Tatyana's notebook](/Sam%27s+Working+Notebook+July-September+2010#vt20080828))

Gigas Control #2 Gill 24E (from [20080828; Tatyana's notebook](/Sam%27s+Working+Notebook+July-September+2010#vt20080828))

NB-1209-10 (RNA Later)

SB-1209-14 (RNA Later)

WB-1209-09 (RNA Later)

0629 gill 5aza

0629 gonad 5aza

0629 mantle 5aza
