---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-05-27 16:33:56+00:00
layout: post
slug: gdna-isolation-mac-gigas-gill-samples-2
title: gDNA Isolation - Mac gigas gill samples
categories:
  - 2010
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNazol
  - Pacific oyster
  - R51
  - RNAlater
---

Set up gDNA isolation from the following samples:

R51 01 (WB R051-0410-01)

R51 11 (WB R051-0410-11)

Samples were thawed from -80C. Tissue was removed from RNA Later ([RNA Later gDNA isolation protocol; this protocol doesn't indicate that anything needs to be done to the sample prior to gDNA isolation](https://www.ambion.com/techlib/misc/genomicDNA_rnalater.html)) and ~25mg was cut from each and placed in 0.5mL of DNAzol. 2.7uL of Proteinase K (Fermentas; 18.5mg/mL) was added to each tube to reach a final concentration of 100ug/mL. The digests were incubated O/N @ RT with rotation.
