--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2010-05-12 16:44:09+00:00 layout: post slug: solid-epcrs-yellow-perch-ct-wb-and-lake-trout-lean-libraries title: SOLiD ePCRs - Yellow perch CT, WB and lake trout Lean libraries categories: - 2010 - Miscellaneous tags: - CT - ePCR - lake trout - Lean - library prep - SOLiD - SOLiD libraries - WB - yellow perch --- Performed ePCRs on these samples from DATE, following the "full scale" protocol. A work flow analysis (WFA) run on these samples from the initial ePCRs/templated bead prep (DATE) revealed too many polyclonal beads, thus requiring them to be redone. ePCRs will be performed using 1.0pM (120pg/uL) of the SOLiD cDNA fragment libraries, instead of the 1.5pM (180pg/uL) used previously. CT - WB - Lean -