---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-05-05 16:46:28+00:00
layout: post
slug: dna-isolation-qiagen-kit-comparison
title: DNA Isolation - Qiagen Kit Comparison
categories:
  - 2010
  - Miscellaneous
tags:
  - DNeasy
  - gDNA
  - gel
  - Hyperladder I
  - troubleshooting
---

Note: This information was added 20140407. Yes, you read that correctly.

Someone had noticed that gDNA isolated using a Qiagen DNeasy Blood & Tissue Kit we rec'd in April 2010 seemed to be yielding degraded DNA.

The two samples used for the comparison were a single tail (split in two equal weight pieces) from a juvenile salmon that was snap frozen, without preservatives, at the time of its collection. The samples were prepped. 0.5ug of eluted DNA was then run on a 1.2% agarose-TAE gel containing 0.1ug/mL of ethidium bromide. 5uL of [Bioline's Hyperladder I](https://www.bioline.com/h_prod_detail.asp?itemid=153) was loaded for size assessment (see link for marker layout).

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/20100505%20Qiagen%20Kit%20compare%20swhite.jpg)

Clearly, there's significant quality difference! A free replacement kit was sent by Qiagen.