---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-04-15 17:06:07+00:00
layout: post
slug: epcr-solid-libraries-lake-trout-sisco-and-herring-go-hpws09-libraries-from-20100408
title: ePCR SOLiD Libraries - Lake Trout Sisco and Herring G/O HPWS09 libraries (from
  20100408)
categories:
  - 2010
  - Miscellaneous
tags:
  - 1 G/O HPWS09
  - ePCR
  - lake trout
  - library prep
  - Pacific herring
  - Sisco
  - siscowet
  - SOLiD
  - SOLiD libraries
---

ePCR was performed for the above three mentioned SOLiD libraries using 1.5pM (180 pg/uL) of cDNA, according to the ABI "full scale" ePCR protocol. ePCRs were stored @ 4C until ready for the emulsion breaking step.

Amounts of cDNA used to make dilutions (in 1x Low TE Buffer) of 180pg/uL:

Sisco (42.29 ng/uL): 2.13uL in 500uL

HPWS09 (9.29 ng/uL): 1.94uL in 100uL