---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-04-07 17:20:07+00:00
layout: post
slug: gel-purification-pcr-cdna-solid-libraries-abalone-yellow-perch-lake-trout-herring
title: Gel Purification & PCR cDNA SOLiD Libraries - Abalone, Yellow Perch, Lake Trout,
  Herring
categories:
  - 2010
  - Miscellaneous
tags:
  - 1 G/O HPWS09
  - black abalone
  - Carmel
  - CC
  - cDNA
  - CE
  - CT
  - gel
  - Haliotis cracherodii
  - in-gel PCR
  - lake trout
  - Lean
  - library prep
  - Pacific herring
  - PQ
  - Sisco
  - siscowet
  - SOLiD
  - SOLiD libraries
  - WB
  - Whole Transcriptome Analysis Kit
  - yellow perch
---

cDNA was gel purified according to Ambion's Whole Transcriptome Analysis Kit. The appropriate regions (100 - 200bp) were excised and cut in to 4, 1x5mm pieces. The two "internal" pieces were transferred to individual PCR tubes. The "outer" pieces were transferred together to a 1.5mL snap cap tube and stored @ -20C.

Three images are below. The first two are the gels before excising the 100 - 200bp region of the gel. The third is the image of the SECOND gel after the specified region was excised. An image was not taken of Gel 1 after excision (whoops!).

Gel 1

![](https://eagle.fish.washington.edu/Arabidopsis/20100407-01.jpg)



Gel 2

![](https://eagle.fish.washington.edu/Arabidopsis/20100407-02.jpg)

NOTE: The WB sample in the gell above is actually a yellow perch sample, NOT an abalone sample!



Gel 2 AFTER EXCISION

![](https://eagle.fish.washington.edu/Arabidopsis/20100407-03.jpg)

NOTE: The WB sample in the gell above is actually a yellow perch sample, NOT an abalone sample!





### In-gel PCR SOLiD Libraries - Abalone, Yellow Perch, Lake Trout, Herring



In-gel PCR was performed on the individual "internal" gel pieces that were excised, as described below from earlier today. PCR rxns/cycling were performed according to Ambion's Whole Transcriptome Analysis Kit. PCR ran O/N. PCR master mix set up is here (bottom half of sheet).