---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-04-06 17:26:25+00:00
layout: post
slug: reverse-transcription-solid-libraries-abalone-yellow-perch-lake-trout-herring
title: Reverse Transcription SOLiD Libraries - Abalone, Yellow Perch, Lake Trout,
  Herring
categories:
  - 2010
  - Miscellaneous
tags:
  - 1 G/O HPWS09
  - black abalone
  - Carmel
  - CC
  - CE
  - CT
  - Haliotis cracherodii
  - lake trout
  - Lean
  - library prep
  - Pacific herring
  - PQ
  - reverse transcription
  - Sisco
  - siscowet
  - SOLiD
  - SOLiD libraries
  - WB
  - Whole Transcriptome Analysis Kit
  - yellow perch
---

Samples were speedvac'd to dryness and resuspended in 3uL of nuclease-free H2O. Samples were then reversed transcribed according to Ambion's Whole Transcriptome Analysis Kit. RT master mix set up is here (top portion of sheet).
