---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-03-25 17:44:50+00:00
layout: post
slug: solid-library-prep-mrna-perch-lake-trout-herring-from-20100318-fragmentation
title: SOLiD Library Prep - mRNA (perch, lake trout, herring from 20100318) Fragmentation
categories:
  - 2010
  - Miscellaneous
tags:
  - 1 G/O HPWS09
  - CT
  - lake trout
  - Lean
  - library prep
  - mRNA
  - Pacific herring
  - PQ
  - Ribominus Concentration Module Kit
  - RNA fragmentation
  - Sisco
  - siscowet
  - SOLiD
  - SOLiD libraries
  - WB
  - Whole Transcriptome Analysis Kit
  - yellow perch
---

Fragmented mRNA according to Ambion's Whole Transcriptome Sequencing Kit. Cleaned up sample using Ribominus Concentration Module (Invitrogen) according to Ambion's WTS Analysis Kit. Samples were eluted w/20uL of H2O and stored @ -80C. Will Bioanalyze and speedvac at a later date.
