---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-01-13 19:33:34+00:00
layout: post
slug: solid-epcrs-herring-cdna-libraries
title: SOLiD ePCRs - Herring cDNA libraries
categories:
  - 2010
  - Miscellaneous
tags:
  - 4LHTOG09
  - 6LHPWS09
  - cDNA
  - ePCR
  - library prep
  - Pacific herring
  - SOLiD
  - SOLiD libraries
---

### Herring fragmented cDNA library: 4LHTOG09



Using 1.5pM of starting template, based on success of the 3LHSITK09 bead prep (see 20100108).

Processed herring fragmented cDNA library 4LHTOG09 (20.1.ng/uL) according to the ABI "Templated Bead Preparation Guide" following the "full-scale" protocol. Made a 1:100 dilution (1uL library, 99uL 1x Low TE) = 201pg/uL. Mixed 89.6uL of this diluted sample with 10.4uL 1x Low TE to get a final concentration of 180pg/uL (1.5pM, according to ABI protocol). Oil phase used was previously prepared by Jesse (Seeb lab) 1/11/2010. This oil phase is stable for 2 months @ 4C.

ePCR was run. The plate will be stored @ 4C until the two remaining libraries have been through ePCR. Then, all three libraries will be processed simulatneously.



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### Herring fragmented cDNA library: 6LHPWS09



Using 1.5pM of starting template, based on success of the 3LHSITK09 bead prep (see 20100108).

Processed herring fragmented cDNA library 6LHPWS09 (51.4.ng/uL) according to the ABI "Templated Bead Preparation Guide" following the "full-scale" protocol. Made a 1:100 dilution (1uL library, 99uL 1x Low TE) = 541pg/uL. Mixed 35uL of this diluted sample with 65uL 1x Low TE to get a final concentration of 180pg/uL (1.5pM, according to ABI protocol). Oil phase used was previously prepared by Jesse (Seeb lab) 1/11/2010. This oil phase is stable for 2 months @ 4C.

ePCR was run. The plate will be stored @ 4C until the two remaining libraries have been through ePCR. Then, all three libraries will be processed simultaneously.