---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2010-01-11 19:43:51+00:00
layout: post
slug: qpcrs-macs-bbdh-cdna-from-20091223
title: qPCRs - Mac's BB/DH cDNA from 20091223
categories:
  - 2010
  - PROPS
tags:
  - BB
  - CALL
  - cDNA
  - CP17A
  - Crassostrea gigas
  - DH
  - EF1
  - GNRR2
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SPI
  - SYTO 13
---

GNRR2 and CALL primer sets used. These are duplicates based on initial differences seen between BB and DH expression. [qPCR set up and plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20100111-03.jpg).

Results:

qPCR Data File (Opticon): [20100111_180230.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20100111_180230.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)



SPI and CP17A primer sets used. These are duplicates based on initial differences seen between BB and DH expression. [qPCR set up and plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20100111-02.jpg).

Results:

qPCR Data File (Opticon): [20100111_141711.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20100111_141711.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)



EF1 primers in duplicate, since I had not done these primers yet. [qPCR set up and plate layout is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20100111-01.jpg).

Results:

qPCR Data File (Opticon): [20100111_102001.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20100111_102001.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)


