---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-31 23:24:05+00:00
layout: post
slug: qpcr-bb-dh-cdna-from-20091223
title: qPCR - BB & DH cDNA (from 20091223)
categories:
  - 2009
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DH
  - GSTO1
  - Opticon2
  - Pacific oyster
  - qPCR
---

qPCR was set up on these cDNAs using the following primers:

FP008495.p.cg.6 ("GSTO1", "Glutathione S-transferase omega-1") - This was upregulated in BB SOLiD data.

These were run in duplicate to take up a full PCR plate. [qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091231-01.jpg).

Results:

qPCR Data File (Opticon): [20091231_152520.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091231_152520.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)