---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-30 23:28:28+00:00
layout: post
slug: qpcrs-bb-dh-cdna-from-20091223
title: qPCRs - BB & DH cDNA (from 20091223)
categories:
  - 2009
  - PROPS
tags:
  - BB
  - Crassostrea gigas
  - DEF1
  - DH
  - Opticon2
  - Pacific oyster
  - qPCR
  - RETST
  - SEMSA
  - TIMP3
---

qPCR was set up on these cDNAs using the following primers:

AJ582629.p.cg.6 ("DEF1", "Defensin 1") - This was upregulated in BB SOLiD data.

CB617519.p.cg.6 ("RETST", "All-trans retinol") - This was upregulated in BB SOLiD data.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091230-01.jpg).

Results:

qPCR Data File (Opticon): [20091230_173747.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091230_173747.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)





qPCR was set up on these cDNAs using the following primers:

CU684779.p.cg.6 ("SEMSA", "Semaphorin-SA") - This was upregulated in BB SOLiD data.

FP004879.p.cg.6 ("TIMP3", "Metalloproteinase inhibitor 3") - This was upregulated in BB SOLiD data.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091230-02.jpg).

Results:

qPCR Data File (Opticon): [20091230_143643.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091230_143643.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)