---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-29 23:35:13+00:00
layout: post
slug: qpcr-bb-dh-cdna-from-20091223-and-emma-primer-sets-for-testing
title: qPCR - BB & DH cDNA (from 20091223) and Emma primer sets for testing
categories:
  - 2009
  - PROPS
tags:
  - BB
  - cDNA
  - Crassostrea gigas
  - DH
  - DPGN1
  - Opticon2
  - Pacific oyster
  - PGSC1
  - qPCR
---

qPCR was set up on these cDNAs using the following primers:

EW778389.p.cg.6 ("DPGN", "Serine protease inhibitor dipetalogastin") - This was upregulated in DH SOLiD data.

FP001672.p.cg.6 ("PGSC1", "Peptidoglycan-recognition protein SC1a/b") - This was upregulated in DH SOLiD data.

Included three primer sets of Emma's (matrillin, beta tub and chaperonin). These were set up with no template and done in duplicate.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091229-01.jpg).

Results:

qPCR Data File (Opticon): [20091229_133148.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091229_133148.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)

Emma's "beta tub" primers show some weird fluorescence, however none of the primer sets show any thing in the melting curve analysis.