---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-29 23:41:52+00:00
layout: post
slug: qpcr-bb-dh-cdna-from-20091223-2
title: qPCR - BB & DH cDNA (from 20091223)
categories:
  - 2009
  - PROPS
tags:
  - BB
  - cDNA
  - Crassostrea gigas
  - DH
  - DJB12
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO 13
  - TOP1
---

qPCR was set up on these cDNAs using the following primers:

FP010108.p.cg.6 ("DJB12", "DnaJ homolog subfamily B member 12") - This was upregulated in DH SOLiD data.

AJ565670.p.cg.6 ("TOP1", "DNA topoisomerase 1") - This was upregulated in BB SOLiD data.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091229-02.jpg).

Results:

qPCR Data File (Opticon): [20091229_164912.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091229_164912.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)