---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-28 23:44:52+00:00
layout: post
slug: qpcrs-bb-dh-cdna-from-20091223-2
title: qPCRs - BB & DH cDNA (from 20091223)
categories:
  - 2009
  - PROPS
tags:
  - BB
  - BDEF
  - CALL
  - CATL
  - cDNA
  - Crassostrea gigas
  - DH
  - GNRR2
  - GSTA
  - Immomix
  - Opticon2
  - qPCR
  - SYTO 13
  - TIMP3
---

qPCR was set up on these cDNAs using the following primers:

AM861391.p.cg.6 ("BDEF", "Big Defensin") - This was upregulated in DH SOLiD data.

AM904566.p.cg.6 ("GNRR2", "Gonadotropin-releasing hormone II receptor") - This was upregulated in DH SOLiD data.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091228-01.jpg).

Results:

qPCR Data File (Opticon): [20091228_102019.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091228_102019.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)





qPCR was set up on these cDNAs using the following primers:

CU988730.p.cg.6 ("TIMP3", "Metalloprotease inhibitor 3") - This was upregulated in DH SOLiD data.

CU990442.p.cg.6 ("CALL", "Calmodulin-like protein") - This was upregulated in DH SOLiD data.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091228-02.jpg).

Results:

qPCR Data File (Opticon): [20091228_135507.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091228_135507.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)





qPCR was set up on these cDNAs using the following primers:

CU994646.p.cg.6 ("CATL", "Cathepsin L") - This was upregulated in DH SOLiD data.

ES789598.p.cg.6 ("GSTA", "Glutathione S-transferase A") - This was upregulated in DH SOLiD data.

[qPCR set up and plate layout can be found here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091228-03.jpg).

Results:

qPCR Data File (Opticon): [20091228_165801.tad](https://eagle.fish.washington.edu/Arabidopsis/qPCR/Opticon/20091228_165801.tad)

Data workup: [PROPS BB_DH Gene Expression Miner Workup](https://docs.google.com/spreadsheet/ccc?key=0AmS_90rPaQMzdHNfWS1oUHUxNFNwci1zcmhhWjhzZnc&usp=sharing)