---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-24 00:01:18+00:00
layout: post
slug: sequencing-mac-methylation-samples-sam-rhodopsin-samples-lisa-samples
title: Sequencing - Mac methylation samples, Sam rhodopsin samples, Lisa samples
categories:
  - 2009
  - Miscellaneous
tags:
  - cuttlefish
  - opsin
  - rhodopsin
  - Sanger sequencing
  - Sepia officinalis
  - Ultrafree-DA
---

Samples were submitted for sequencing. Mac prepped all Roberts Lab samples excluding the Opsin VMC gel slice 2 from 20091217-02. The gel slice was purified with Millipore spin columns. The sample was diluted 1:1 with H2O and submitted for sequencing, one time from each direction using the Sep_Op_Fw2/Rv2 primers. [Plate layout can be found here on sheet labeled "20091223"](https://spreadsheets.google.com/ccc?key=0AtV_gF766XZAcHljOFBWd3pLTUJwbUxkdkg1OGdCY3c&hl=en).