---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-09 00:34:55+00:00
layout: post
slug: rna-adapter-hybridization-and-ligation-herring-liver-mrna-for-solid-libraries
title: RNA Adapter Hybridization and Ligation - Herring Liver mRNA for SOLiD Libraries
categories:
  - 2009
  - Miscellaneous
tags:
  - 2L
  - 3L
  - 4L
  - 6L
  - hybridization
  - library prep
  - ligation
  - liver
  - mRNA
  - Pacific herring
  - SOLiD
  - SOLiD libraries
  - Whole Transcriptome Analysis Kit
---

RNA from yesterday was speedvac'd to dryness and resuspended in 3uL of nuclease-free H2O. Samples were mixed with Adaptor Mix A and hybridized according to Ambion WTK protocol. Samples were then ligated for 16hrs @ 16C, according to Ambion WTK protocol.
