---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-12-02 01:15:02+00:00
layout: post
slug: rna-precipitation-herring-liver-rna-for-solid-libraries
title: RNA Precipitation - Herring Liver RNA for SOLiD Libraries
categories:
  - 2009
  - Miscellaneous
tags:
  - 2L
  - 2L HKOD09
  - 3L
  - 3L HSITK09
  - 4L
  - 4L HTOG09
  - 6L
  - 6L HPWS09
  - EtOH precipitation
  - library prep
  - liver
  - Pacific herring
  - RNA
  - SOLiD
  - SOLiD libraries
---

50uL of RNA from each of the following were precipitated O/N @ -20C:

2L HKOD09 - 2.157 ug/uL x 50uL = 107.85ug

4L HTOG09 - 2.089 ug/uL x 50uL = 104.45ug

3L HSITK09 - 1.089 ug/uL x 50uL = 54.45ug

6L HPWS09 - 1.703 ug/uL x 50uL = 85.15ug

0.1 volumes (5uL) of 3M NaOAc ph = 5.2 was added to each sample. Then 2 volumes (110uL) of 100% EtOH was added. Samples were vortexed and incubated O/N @ -20C.
