---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-28 02:30:27+00:00
layout: post
slug: mrna-isolation-herring-gonadovary-rna-from-20091023
title: mRNA Isolation - Herring gonad/ovary RNA (from 20091023)
categories:
  - 2009
  - Miscellaneous
tags:
  - EtOH precipitation
  - gonad
  - Micro PolyA Purist
  - mRNA
  - mRNA enrichment
  - NanoDrop1000
  - ovary
  - Pacific herring
  - RNA quantification
---

#### RNA Precipitation



Sample was spun 16,000g, 30mins, 4C. Supe removed. Pellet washed with 1mL 70% EtOH. Spun 16,000g, 10mins, 4C. Supe removed. Pellet resuspended in 250uL of nuclease free H2O. Will proceed with mRNA isolation.





#### mRNA Isolation



Isolated mRNA using Ambion's MicroPolyA Purist Kit according to protocol. Performed two rounds of isolation to decrease residual rRNA carryover that we frequently see after a single round.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20091027%20mRNA%20SJW.jpg)

Started with ~90ug of total RNA. Yield of mRNA = 3.26ug. That is a ~3.6% recovery of mRNA.
