---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-27 03:16:30+00:00
layout: post
slug: mrna-isolation-herring-liver-rna-from-20091021
title: mRNA Isolation - Herring Liver RNA (from 20091021)
categories:
  - 2009
  - Miscellaneous
tags:
  - liver
  - Micro PolyA Purist
  - mRNA
  - mRNA enrichment
  - NanoDrop1000
  - Pacific herring
  - RNA quantification
---

Isolated mRNA using Ambion's MicroPolyA Purist Kit according to protocol. Performed two rounds of isolation to decrease residual rRNA carryover that we frequently see after a single round.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20091026%20mRNA%20SJW.jpg)

Started with ~500ug. Total yield = 5.3ug. That is a 1.06% recovery of mRNA.
