---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-24 03:20:40+00:00
layout: post
slug: rna-isolation-herring-gonadovary-samples
title: RNA Isolation - Herring Gonad/Ovary Samples
categories:
  - 2009
  - Miscellaneous
tags:
  - gonad
  - NanoDrop1000
  - ovary
  - Pacific herring
  - RNA isolation
  - RNA quantification
  - TriReagent
---

RNA was isolated according to protocol. Pellets were resuspended in 50uL of 0.1%DEPC-H2O, heated @ 55C for 5 mins, spec'd and stored @ -80C in the "Herring RNA Box #1".

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20091023%20RNA%20SJW.jpg)

Most of the samples look good, however there are a number of samples that are downright bad. Either no RNA or very low concentrations with poor 260/280, 260/230 ratios.
