---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-23 03:23:44+00:00
layout: post
slug: rna-isolation-herring-gonadovary-samples-2
title: RNA Isolation - Herring Gonad/Ovary Samples
categories:
  - 2009
  - Miscellaneous
tags:
  - gonad
  - ovary
  - Pacific herring
  - RNA
  - RNA isolation
  - TriReagent
---

From the Seeb Lab. Homogenized entire gonad/ovary samples in 5mL of TriReagent with the sonicator. In essence, based on the manufacturer's recommendation, this means the ratio of tissue:TriReagent was ~2x. Transferred 0.5mL of homogenized gonad/ovary sample to 1.5mL snap cap tubes and added an additional 0.5mL of TriReagent, to adjust the ratio of tissue:TriReagent to ~1x. Samples were then stored @ -80C. These will be further processed tomorrow.
