---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-21 03:29:54+00:00
layout: post
slug: rna-isolation-herring-liver-samples-lhpws09-1-6
title: RNA Isolation - Herring Liver Samples (LHPWS09 1-6)
categories:
  - 2009
  - Miscellaneous
tags:
  - liver
  - Pacific herring
  - RNA
  - RNA isolation
  - TriReagent
---

From Seeb Lab. Homogenized entire liver samples in 5mL of TriReagent with the Tissue Tearers. In essence, based on the manufacturer's recommendation, this means the ratio of tissue:TriReagent was ~2x. Transferred 0.5mL of homogenized liver sample to 1.5mL snap cap tubes and added an additional 0.5mL of TriReagent, to adjust the ratio of tissue: TriReagent to ~1x. Samples were then stored @ -80C. These will be further processed once all remaining liver samples have been homogenized inTriReagent.
