---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-13 03:35:18+00:00
layout: post
slug: qpcr-tims-adult-gigas-challenge-cdna-from-20091009
title: qPCR - Tim's adult gigas challenge cDNA (from 20091009)
categories:
  - 2009
  - Miscellaneous
tags:
  - cDNA
  - Crassostrea gigas
  - expression analysis
  - graphs
  - HIF1
  - Immomix
  - Pacific oyster
  - Postaglandin E2
  - qPCR
  - SYTO 13
  - Vibrio tubiashii
---

Set up qPCR with Cg_HIF1 (hypoxia induced factor 1) primers and prostaglandin E2 primers. [Plate layout/setup is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091012-01.jpg).

Results: Processed with PCR Miner. Normalized to EF1. Standard Error bars. Here is spreadsheet with workup.

![](https://eagle.fish.washington.edu/Arabidopsis/20091012%20Adults%20HIF1%20graph.jpg)![](http://eagle.fish.washington.edu/Arabidopsis/20091012%20Adults%20PgE2%20graph.jpg)

**HIF1**:

No significant differences between any treatments.

**Prostaglandin E2**:

No significant differences between any treatments.
