---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-10 03:44:54+00:00
layout: post
slug: reverse-transcription-tims-adult-gigas-challenge-dnased-rna-from-20091008
title: Reverse Transcription - Tim's adult gigas challenge DNased RNA (from 20091008)
categories:
  - 2009
  - Miscellaneous
tags:
  - cDNA
  - Crassostrea gigas
  - DNased RNA
  - M-MLV
  - oligo dT
  - Pacific oyster
  - reverse transcription
---

Performed RT rxns. [Setup is here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091009-02.jpg). cDNA was subsequently diluted 1:4 (added 75uL H2O to each well) to properly mirror Tim's previous qPCRs.

UPDATE: cDNA plate was discarded 20120320 by SJW.
