---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-09 03:53:17+00:00
layout: post
slug: qpcr-tims-adults-gigas-challenge-dnased-rna-from-today
title: qPCR - Tim's adults gigas challenge DNased RNA (from today)
categories:
  - 2009
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNased RNA
  - EF1
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO 13
---

Performed qPCR using q18s primers on DNased RNA (1:100 dilution to match final concentration of template after making cDNA). [qPCR set up and plate layout are here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091008-01.jpg).

gDNA dilutions were used as positive controls. gDNA = BB11 (0.49ug/uL) from [20090519](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090519). Used 5uL of 1:10, 1:100 and 1:000 dilutions.

Results: All samples, including positive controls, came up negative! Realized that I accidentally used the EF1 primers which will NOT amplify gDNA. And, to top it off, this was BEFORE going to seminar (i.e. before having any beers).