---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-09 03:56:16+00:00
layout: post
slug: qpcr-tims-adults-gigas-challenge-dnased-rna-from-today-2
title: qPCR - Tim's adults gigas challenge DNased RNA (from today)
categories:
  - 2009
  - Miscellaneous
tags:
  - Crassostrea gigas
  - DNased RNA
  - EF1
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO 13
---

Previous qPCR was done incorrectly (wrong primers), so am repeating with the correct primers. Performed qPCR using q18s primers on DNased RNA (1:100 dilution to match final concentration of template after making cDNA). [qPCR set up and plate layout are here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091008-02.jpg).

gDNA dilutions were used as positive controls. gDNA = BB11 (0.49ug/uL) from [20090519](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090519). Used 5uL of 1:10, 1:100 and 1:000 dilutions.

Results: Still had 5 samples that came up positive. Will re-DNase treat these samples and then re-qPCR them.