---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-07 04:05:27+00:00
layout: post
slug: reverse-transcriptioncdna-purificationemulsion-pcr-ligation-rxns-of-trout-fragmented-rna-for-solid-wtk-from-yesterday
title: Reverse Transcription/cDNA purification/Emulsion PCR - Ligation rxns of trout
  fragmented RNA for SOLiD WTK (from yesterday)
categories:
  - 2009
  - Miscellaneous
tags:
  - cDNA
  - DNA Quantification
  - ePCR
  - ligation
  - NanoDrop1000
  - RBC
  - reverse transcription
  - RNA
  - SOLiD
  - SOLiD libraries
  - trout
  - Whole Transcriptome Analysis Kit
---

The four samples from yesterday were prepared according to the Agilent SOLiD WTK protocol. Briefly:





Results: All four samples appear to have cDNA. Interestingly, the "Amped cDNA trout RBC control ribo(-)" sample was the sample that had no detectable RNA after fragmentation, BUT this sample produced the highest yield of cDNA... See below.

1.5uL of each sample was transferred to a 0.5mL snap cap tube and stored @ -80C in the "Samples for Bioanalyzer" box for submission on the DNA 1000 Chip.

![](https://eagle.fish.washington.edu/Arabidopsis/20091006%20LT%20RBC%20amped%20cDNA%20SJW-01.jpg)

![](https://eagle.fish.washington.edu/Arabidopsis/20091006%20LT%20RBC%20amped%20cDNA%20SJW-02.jpg)

The Yellow/Brown plot above is the "Amped cDNA trout RBC poly I:C ribo(-) & polyA" sample and exhibits a strange profile at the 220-230nm range that differs than the three other samples.