---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-06 04:09:07+00:00
layout: post
slug: qpcr-tims-adults-gigas-challenge-dnased-rna-from-20091002
title: qPCR - Tim's adults gigas challenge DNased RNA (from 20091002)
categories:
  - 2009
  - Miscellaneous
tags:
  - 18s
  - Crassostrea gigas
  - DNased RNA
  - Immomix
  - Opticon2
  - Pacific oyster
  - qPCR
  - SYTO 13
---

Performed qPCR using q18s primers on DNased RNA (1:100 dilution to match final concentration of template after making cDNA). [qPCR set up and plate layout are here](https://eagle.fish.washington.edu/Arabidopsis/Notebook%20Workup%20Files/20091005-01.jpg).

gDNA dilutions were used as positive controls. gDNA = BB11 (0.49ug/uL) from [20090519](/Sam%27s+Working+Notebook+Jan-May+2009#sjw20090519). Used 5uL of 1:10, 1:100 and 1:000 dilutions.

Results: gDNA dilutions look good. However, some samples are definitely coming up before the 40 cycle mark. Will re-DNase treat these.