---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-06 04:11:55+00:00
layout: post
slug: adapter-ligation-ricks-trout-fragmented-controlpoly-ic-samples-for-solid-wtk
title: Adapter Ligation - Rick's trout fragmented control/poly I:C samples for SOLiD
  WTK
categories:
  - 2009
  - Miscellaneous
tags:
  - library prep
  - ligation
  - RBC
  - SOLiD
  - SOLiD libraries
  - trout
  - Whole Transcriptome Analysis Kit
---

See the Next Gen Seq Library Database for more info. Processed the 4 samples (one set Ribominus only, one set Ribominus + PolyA enriched) according to the Agilent WTK. Briefly:




    
  * Speedvac'd samples to dryness

    
  * Resuspended RNA in 3uL H2O

    
  * Adapter rxn. Used all 3uL of RNA (used only 1uL of RBC Ribo only sample due to high concentration)

    
  * Ligation rxn



Incubated 16C for 16hrs.