---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-10-01 04:18:34+00:00
layout: post
slug: rna-isolation-tims-adult-gigas-challenge-samples
title: RNA Isolation - Tim's adult gigas challenge samples
categories:
  - 2009
  - Miscellaneous
tags:
  - Crassostrea gigas
  - gill
  - Pacific oyster
  - RNA isolation
  - RNA quantification
  - TriReagent
  - Vibrio tubiashii
---

RNA was isolated using 500uL of TriReagent for all samples. Samples were resuspended in 100uL of 0.1%DEPC-H2O and spec'd. Samples stored in Tim's "NAME OF BOX" box.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090930%20RNA%20SJW.jpg)

AC# = Air Control Sample

CC# = CO2 Control Sample

AV# = Air Vibrio Sample

CV# = CO2 Vibrio Sample

All the samples look really good, even those that exhibited dark coloration carried over from extraction. Will check for gDNA contamination.