---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-09-29 04:34:51+00:00
layout: post
slug: etoh-precipitation-ricks-trout-ribosomoal-depleted-rna-for-solid-wtk-from-today
title: EtOH Precipitation - Rick's trout Ribosomoal-depleted RNA for SOLiD WTK (from
  today)
categories:
  - 2009
  - Miscellaneous
tags:
  - EtOH precipitation
  - library prep
  - mRNA
  - RBC
  - RNA
  - SOLiD
  - SOLiD libraries
  - trout
---

The "control" and "poly I:C" samples prepared earlier today were EtOH precipitated in preparation for fragmentation.

Added the following to each sample:




    
  * 18uL 5M ammonium acetate

    
  * 1uL glycogen

    
  * 2 vols. of 100% EtOH (74uL)



Samples were incubated O/N @ -80C.
