--- author: Sam White toc-title: Contents toc-depth: 5 toc-location: left date: 2009-09-29 04:34:51+00:00 layout: post slug: etoh-precipitation-ricks-trout-ribosomoal-depleted-rna-for-solid-wtk-from-today title: EtOH Precipitation - Rick's trout Ribosomoal-depleted RNA for SOLiD WTK (from today) categories: - 2009 - Miscellaneous tags: - EtOH precipitation - library prep - mRNA - RBC - RNA - SOLiD - SOLiD libraries - trout --- The "control" and "poly I:C" samples prepared earlier today were EtOH precipitated in preparation for fragmentation. Added the following to each sample: * 18uL 5M ammonium acetate * 1uL glycogen * 2 vols. of 100% EtOH (74uL) Samples were incubated O/N @ -80C.