---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-09-24 04:38:16+00:00
layout: post
slug: rna-fragmentation-ricks-trout-rbc-samples-prepped-earlier-today-see-below
title: RNA Fragmentation - Rick's trout RBC samples prepped earlier today (see below)
categories:
  - 2009
  - Miscellaneous
tags:
  - library prep
  - NanoDrop1000
  - RBC
  - Ribominus Concentration Module Kit
  - RNA fragmentation
  - RNA quantification
  - SOLiD
  - SOLiD libraries
  - trout
  - Whole Transcriptome Analysis Kit
---

Samples were fragmented according to the Whole Transcriptome Kit protocol. Samples were then cleaned up using Invitrogen's Modified RiboMinus Concentration Module, according to protocol. Briefly:




    
  * Added 1X volume of binding buffer (100uL)

    
  * Added equal volume of 100% EtOH so that [EtOH] = 50% (200uL)



Followed remainder of protocol and eluted with 20uL of H2O. Samples were spec'd.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090923%20fragged%20trout%20RBC%20ribo-%20polyA%20RNA%20SJW.jpg)

Assuming the NanoDrop readings are accurate (according to the Whole Transcriptome Kit, these may NOT be accurate), got yields of ~93ng for the RBC Control sample and ~132ng for the RBC poly1:C sample. Transferred 1.5uL of each sample to separate 0.5mL tubes for submission to the Bioanalyzer. These were stored @ -80C in the "Bioanalyzer Samples" box. The remainder of the samples were stored @ -80C in the "Samples from Other Researchers" box.
