---
author: Sam White
toc-title: Contents
toc-depth: 5
toc-location: left
date: 2009-09-24 04:36:22+00:00
layout: post
slug: mrna-isolation-ricks-trout-rbc-samples-previously-treated-with-ribominus-kit-by-mac
title: mRNA Isolation - Rick's trout RBC samples previously treated with Ribominus
  Kit (by Mac)
categories:
  - 2009
  - Miscellaneous
tags:
  - EtOH precipitation
  - Micro PolyA Purist
  - mRNA
  - mRNA enrichment
  - NanoDrop1000
  - RBC
  - RNA quantification
  - trout
---

Was given ~0.5ug of each of these two RNA samples and processed them with Ambion's microPolyA Purist Kit according to protocol. After elution, the samples were EtOH precipitated @ -80C for 30mins, pelleted 30mins 16,000g for 30mins, 4C. Supe removed, RNA washed with 1mL 70% EtOH and spun 10mins 16,000g, 4C. Supe removed. Resusupended in 8uL of The RNA Storage Solution and spec'd.

Results:

![](https://eagle.fish.washington.edu/Arabidopsis/RNA%20Spec%20Readings/20090923%20trout%20ribo-%20polyA%20RNA%20SJW.jpg)

Yield of ~320ng for RBC Control sample and ~360ng for RBC poly1:C sample. Will proceed to Whole Transctiptome Kit fragmentation step.
